December
8th,
2012
My last publication while working at the ESRF is finally
out:
|
![[HTML version]](http://journals.iucr.org/s/graphics/htmlborder.gif) ![[PDF version]](http://journals.iucr.org/s/graphics/pdfborder.gif) ![[Supplementary Material]](http://journals.iucr.org/s/graphics/supplementarymaterialsborder.gif) ![[Buy article online]](http://journals.iucr.org/logos/buy.gif) J. Synchrotron Rad. (2013). 20 [ doi:10.1107/S0909049512049114 ] A survey of global radiation damage to 15 different protein crystal types at room temperature: a new decay modelR. M. Ferraz Leal, G. Burenkov, S. Russi and A. N. PopovSynopsis: A systematic study of the sensitivity to radiation damage of crystals held at room temperature for a large set of model macromolecular structures is presented.Online 6 December 2012 |
Abstract: The radiation damage rates to crystals of 15 model macromolecular structures were studied
using an automated radiation sensitivity characterization procedure. The diffracted intensity variation with dose is
described by a two-parameter model. This model includes a strong resolution-independent decay specific to
room-temperature measurements along with a linear increase in overall Debye-Waller factors. An equivalent
representation of sensitivity via a single parameter, normalized half-dose,
is introduced. This parameter varies by an order of magnitude between the different structures studied. The data
show a correlation of crystal radiation sensitivity with crystal solvent content but no dose-rate dependency was
detected in the range 0.05-300 kGy s-1. The results of the crystal
characterization are suitable for either optimal planning of room-temperature data collection or in situ crystallization plate screening experiments.